Isolation and Characterization of Bliai, an Isoschizomer of Clai from Bacillus Licheniformis
نویسندگان
چکیده
The restriction endonuclease BliAI, an isoschizomer of ClaI, which recognizes the sequence 5 ́AT↓CGAT 3 ́, was purified from a natural isolate identified as Bacillus licheniformis. The restriction endonuclease was isolated from cell extracts using single-step purification by phosphocellulose column chromatography. The restriction endonuclease is active at 37oC and over a wide range of pH and salt concentration. The molecular weight of the purified restriction enzyme is consistent with a value of 39 kDa.
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